Overview of single-cell analysis using Seurat

Seurat is a powerful R based scRNAseq analysis package designed for QC, analysis, and exploration of single-cell RNA-seq data. Seurat enables users to perform batch correction integration and identify and interpret sources of heterogeneity from single-cell transcriptomic measurements.

Figure adapted from Butler et al., 2018: Nature Biotechnology

In this course we will take a PBMC dataset derived from Kang et al., 2017: Nature Biotechnology and perform basic sequential QC, integration and DE analysis using the Seurat integration pipeline.

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